This was a very informative! I was familiar with NAO, but I had no idea how low the RNA concentration is of respiratory viruses in wastewater, and how much that drives up the cost of sequencing. How do indoor aerosol samples compare? Seems like air sampling of indoor public spaces might be promising, I found a couple papers demonstrating that detecting pathogens via airborne MGS sequencing is possible but none gave relative abundance numbers.
This was a very informative! I was familiar with NAO, but I had no idea how low the RNA concentration is of respiratory viruses in wastewater, and how much that drives up the cost of sequencing. How do indoor aerosol samples compare? Seems like air sampling of indoor public spaces might be promising, I found a couple papers demonstrating that detecting pathogens via airborne MGS sequencing is possible but none gave relative abundance numbers.