Thanks for sharing the pricing you’ve been getting!
One thing that makes this a bit confusing is that in the context of paired end reads, “read” can either mean a single forward or reverse read, or it can mean a read pair (a gapped read). As we note under Table 3, we’re using the latter: each read is (roughly) an independent observation from the sample. Illumina uses the former, though, and maybe you are as well? In which case instead of $1.3k per billion reads (6x cheaper) you paid $2.6k (3x cheaper)?
> if you’re trying to detect truly novel viruses, using a Kraken database made from existing viral sequences is not going to work!
Definitely! The work in this report wasn’t to identify novel viruses, it was to understand how much sequencing we might need to do to get some number of reads of a novel virus. As a step toward that longer term goal.
> Recently I paid $13,000 for 10 billion reads
Thanks for sharing the pricing you’ve been getting!
One thing that makes this a bit confusing is that in the context of paired end reads, “read” can either mean a single forward or reverse read, or it can mean a read pair (a gapped read). As we note under Table 3, we’re using the latter: each read is (roughly) an independent observation from the sample. Illumina uses the former, though, and maybe you are as well? In which case instead of $1.3k per billion reads (6x cheaper) you paid $2.6k (3x cheaper)?
> if you’re trying to detect truly novel viruses, using a Kraken database made from existing viral sequences is not going to work!
Definitely! The work in this report wasn’t to identify novel viruses, it was to understand how much sequencing we might need to do to get some number of reads of a novel virus. As a step toward that longer term goal.
This was per 10 billion pairs (so 6x cheaper). The specifications are here: https://www.illumina.com/systems/sequencing-platforms/novaseq-x-plus/specifications.html
Thanks!