Comparing agnostic metagenomic sequencing and massively multiplexed bait capture sequencing for clinical disease surveillance and early-warning systems
This will likely be part of my lit review of my master’s thesis and should also make an interesting blog post.
I am unsure whether to call it massively parallelised or massively multiplexed bait capture sequencing when you use on the order of 105-106 probes at the same time
Comparing agnostic metagenomic sequencing and massively multiplexed bait capture sequencing for clinical disease surveillance and early-warning systems
This will likely be part of my lit review of my master’s thesis and should also make an interesting blog post.
I am unsure whether to call it massively parallelised or massively multiplexed bait capture sequencing when you use on the order of 105-106 probes at the same time
What is agnostic metagenomic sequencing? https://doi.org/10.1128/cmr.00119-22
What is massively multiplexed bait capture sequencing? https://doi.org/10.1128/jcm.00612-23
What are the pros and cons of the different approaches?
cost?
amount of labor needed?
how quickly do you get results?
...
examples of Pros of bait capture sequencing
“results in two to three orders of magnitude enhancement in sensitivity at a reduced cost” (Kapoor et al. 2023)
“mitigates Health Insurance Portability and Accountability Act (HIPAA) concerns because host sequence data are not collected” (Kapoor et al. 2023)
How are these methods different from similar, commercially available products?
(e.g., https://www.twistbioscience.com/products/ngs/fixed-panels/comprehensive-viral-research-panel)